Description
- This strain is EndA- and yields high quality DNA preparations.
- The RecA- mutation in XJa stabilizes repetitive DNA sequences.
- Suitable for general screening, but proteases may degrade small or otherwise unstable recombinant proteins.
- Fast: 80 – 90% of E.coli are lysed in only 10 minutes after harvesting.
- Simple 20 Second Transformation: No heat shock! Just add DNA and spread.
- DE3 Lysogen: Encodes the T7 polymerase for expressing recombinant proteins under the control of the T7 promoter.
- 10^8 – 10^9 transformants per µg of plasmid DNA.





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