Biotage Isolute C18EC Chromatography Columns 2 ml 100 mg Each Tube – 100 Columns

Description

Biotage 221-0100-T ISOLUTE C18(EC) SPE Column – 2 mL, 100 mg

The Biotage 221-0100-T is an ISOLUTE C18(EC) Solid Phase Extraction (SPE) column configured in a standard 2 mL syringe format. Each tube contains 100 mg of a silica-based sorbent covalently bonded with octadecyl (C18) hydrocarbon chains. The sorbent undergoes an exhaustive end-capping process to neutralize residual surface silanols, suppressing secondary polar interactions. This non-polar, reversed-phase chemistry is engineered for the extraction and concentration of non-polar to moderately polar analytes from aqueous matrices.

Technical Performance and Design

• End-Capped Sorbent: Exhaustive end-capping minimizes secondary cation-exchange interactions with basic analytes, preventing irreversible binding and ensuring high elution recoveries.

• Reversed-Phase Mechanism: Utilizes strong hydrophobic interactions to retain organic compounds from polar sample matrices such as water, plasma, or urine.

• Silica Substrate: The standardized particle size and pore geometry (typically 50 µm / 60 Å) ensure consistent liquid flow rates when processed on standard vacuum manifolds or positive pressure systems.

• 2 mL Column Format: The 2 mL reservoir accommodates small aqueous sample volumes while utilizing a minimal 100 mg sorbent bed, drastically reducing the volume of organic solvent required for the final elution step.

Product Specifications

• Catalog Number: 221-0100-T

• Product Line: ISOLUTE C18(EC) (End-Capped)

• Format: 2 mL SPE Column (Syringe Format)

• Sorbent Mass: 100 mg per tube

• Retention Mechanism: Non-polar (Hydrophobic)

• Target Analytes: Non-polar and moderately polar compounds

Key Benefits & Applications

• Environmental Analysis: Used for the extraction and concentration of trace-level contaminants, such as pesticides, herbicides, and polycyclic aromatic hydrocarbons (PAHs), from surface and drinking water.

• Bioanalytical Testing: Isolates pharmaceutical drugs, metabolites, and endogenous biomarkers from physiological fluids prior to LC-MS/MS or GC-MS analysis.

• Aqueous Desalting: Effective for removing salts and polar interferences from aqueous sample mixtures before downstream mass spectrometry or lyophilization.

• Solvent Efficiency: The compact 100 mg bed mass reduces solvent consumption during the conditioning, washing, and elution phases, shortening subsequent evaporation and dry-down times.

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