Description
Description
TWEEN 20 Viscous Liquid 4 Liter
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
- CAS Number 9005-64-5
- MDL number MFCD00165986
- NACRES NA.21
| Quality Level | 300 |
| description | non-ionic |
| assay | ≥40.0% (GC) |
| form | viscous liquid |
| mol wt | ~1228 |
| composition | lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids) |
| application(s) | DNA extraction: suitable |
| RNA extraction: suitable | |
| cell culture | mammalian: suitable | |
| protein purification: suitable | |
| impurities | ≤3.0% water |
| refractive index | n20/D 1.468 (lit.) |
| CMC | 0.06 mM (20-25°C) |
| acid number | ≤2.2 mg/g |
| hydroxyl value | 96‑108 mg/g |
| transition temp | cloud point 76 °C |
| density | 1.095 g/mL at 25 °C (lit.) |
| HLB | 16.7 |
| suitability | complies for IR spectroscopy |
General description
Tween20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Documents
Safety Data Sheet
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