Description
Description
Agarose Bioreagent For Molecular Biology Low EEO, 250 Gram
- CAS Number 9012-36-6
- EC Number 232-731-8
- MDL number MFCD00081294
- PubChem Substance ID 24891469
- NACRES NA.25
| Quality Level | 200 |
| biological source | algae (marine) |
| grade | for molecular biology |
| product line | BioReagent |
| form | powder |
| application(s) | electrophoresis: suitable |
| impurities | ≤10% moisture content |
| EEO | 0.09-0.13 |
| transition temp | gel point 36 °C (1.5% gel, ± 1.5 °C) |
| gel strength | ≥1200 g/cm2 (1% gel) |
| anion traces | sulfate (SO42-): ≤0.15% |
| foreign activity | DNase, RNase, none detected |
General description
Agar belongs to the galactan polysaccharides, which make up the intracellular matrix in red seaweeds/marine algae belonging to class Rhodophyta. Agar is naturally slightly sulfated, and is a complex mixture of polysaccharides. Agarose is a neutral polymer component of agar. Heating of agarose gives rise to solution form which on cooling sets into gel form. Gel formation occurs due to double helical formation from molecular chains, which forms a water-immobilizing network.
Application
Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.
Safety Information
Personal Protective Equipment Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport
WGK Germany 3
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