(42-702DOS) Apex 5X cDNA Synthesis Supermix First-strand cDNA synthesis 500 x 20ul Reactions/Unit

Description

• Simple, fast, and sensitive first-strand cDNA synthesis kit for RT-qPCR or RT-PCR
• Preblended 5X supermix contains all the necessary components except RNA template for reverse transcription
• Enhanced, engineered MMLV Rtase has reduced RNase H activity and contains a fidelity-enhancing subunit for superior accuracy
• Potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
• Contains optimized blend of oligo(dT) and random primers for complete RNA coverage
• Complete cDNA synthesis and PCR the same day with 15 minute (for qPCR) or 50 minute (for PCR) cDNA synthesis protocol
• Both poly(A) + mRNA and total RNA can be used for first-strand cDNA synthesis, but poly(A) + mRNA may give higher yields and improved purity