Fisher Scientific Phosphate Buffered Saline 10X Solution 20 L Polycube

Description

Fisher BioReagents Phosphate Buffered Saline (PBS) 10X Solution – 20 L Structural Polycube, Molecular Biology Grade, Concentrated Fluidic Buffering System (MPN: BP399-20)

The Fisher BioReagents BP399-20 is an instrument-grade, highly concentrated (10X) Phosphate Buffered Saline (PBS) liquid solution engineered for large-scale cell culture, molecular biology, tissue harvesting, and general cleanroom diagnostic workflows. Formulated using ultra-pure, molecular biology-grade reagents dissolved in high-purity deionized water, this solution is filtered down to 0.2 microns to eliminate microscopic particulates and baseline microbial contamination. Packaged in a heavy-duty, high-density 20 Liter (20 L) rigid polycube container equipped with a secure, integrated dispensing tap spigot, it provides a stable, long-lasting reservoir for high-throughput labs. When diluted with high-purity water to a 1X working concentration, it yields a physiological buffer matching cellular osmolarity, ensuring optimal cell viability, uniform wash cycles, and steady pH control across sensitive biological assays.

Technical Performance and Design

• Molecular Biology-Grade Purity Matrix: Extensively tested for the presence of common microfluidic contaminants. Certified DNase-free, RNase-free, protease-free, and low in endotoxins, preventing unwanted enzymatic degradation or background interference during high-sensitivity genomic and proteomic procedures.

• High-Density Space-Saving Polycube: Housed inside a rigid, heavy-walled 20 L polycube container. The block geometry optimizes shelf space, while the integrated lower dispensing spigot facilitates controlled, drip-free fluid transfers into secondary carboys or flasks without requiring tipping.

• Physiological Osmolarity Maintenance: When diluted to a 1X working state, the buffer maintains a balanced electrolyte matrix. This duplicates natural physiological osmotic pressures, preventing cell lysis, wrinkling, or structural swelling during cell isolation and washing passes.

• Strict 10X Concentration Calibration: Manufactured to exact electrochemical tolerances, providing a 10-fold concentrated solution of 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.119M Phosphate buffer components for efficient batch preparation.

Product Specifications

• Catalog Number / MPN: BP399-20 (Fisher BioReagents Core Line)

• Manufacturer / Brand: Fisher Scientific / Fisher BioReagents

• Bulk Liquid Volume: 20 Liters (20 L / ~5.28 Gallons)

• Chemical Formulation: 10X Concentrated Phosphate Buffered Saline (PBS) Liquid

• Target Dilution pH: 1X solution yields a highly stable pH of 7.40 ± 0.05 at a room temperature of 25°C

• Filtration Threshold: 0.2-micron sterile-filtered fluid path

• Container Type Profile: Rigid, self-supporting Polyethylene Cube (Polycube) with an integrated dispensing handle and valve spigot assembly

Key Benefits and Applications

• High-Volume Cell Culture Washing: The standard choice for tissue culture core facilities to safely wash cell pellets, rinse growth surfaces, and dilute biological fractions before downstream analysis.

• Immunoassay & ELISA Monoclonal Prep: Serves as an excellent, high-purity buffer base for antibody dilutions, plate-blocking sequences, and micro-well washing loops, ensuring high signal-to-noise ratios.

• Western Blotting Membrane Isolation: Widely deployed to mix custom washing reagents (such as PBST) for removing unbound reagents from nitrocellulose or PVDF transfer membranes.

• Eliminated Powder Mixing Failures: Pre-mixed liquid concentrates eliminate the dust inhalation risks, validation inconsistencies, and heating delays associated with mixing powder salts by hand.

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