Description
Description
Bovine Serum Albumin Heat Shock Fraction pH 7 ≥98%, 1 Kg
Synonym: Albumin bovine serum, BSA, Bovine albumin
- CAS Number 9048-46-8
- EC Number 232-936-2
- MDL number MFCD00130384
- NACRES NA.27
| Quality Level | 200 |
| biological source | bovine |
| assay | ≥98% |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| purified by | heat shock fractionation |
| origin | USA origin |
| application(s) | immunocytochemistry: suitable |
| loss | ≤5% |
| pH | 7 |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
General description
Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
Application
Bovine Serum Albumin is used for many applications, including the following applications:
- BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
- BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
- BSA has been used as a standard in the Bradford method of protein quantification.
- BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.
Safety Information
Personal Protective Equipment Eyeshields, Gloves, type N95 (US)
RIDADR NONH for all modes of transport
WGK Germany 3
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