Description
Invitrogen WA0999 Midi Gel Adapter – For Running Novex Midi Gels in Bio-Rad Criterion Cells
The Invitrogen WA0999 Midi Gel Adapter is a specialized, self-adhesive interface designed to bridge the compatibility gap between different electrophoresis manufacturers. Specifically, it allows Novex Midi Gel Cassettes (including NuPAGE Bis-Tris and Tris-Glycine formats) to be utilized within the Bio-Rad Criterion Cell tank. The adapter features a pressure-sensitive acrylic adhesive and two precision alignment tabs that lock onto the slots of the midi cassette. Once applied, the adapter forms a 75 mL upper buffer chamber, providing the necessary seal to maintain the voltage and buffer levels required for successful protein separation. This disposable, single-use tool ensures that laboratories can standardize their protein electrophoresis protocols on Novex gel chemistry while utilizing their existing Bio-Rad hardware investment.
Technical Performance and Design
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Adhesive-Seal Technology: Features a high-strength, acrylic-based adhesive liner that creates a leak-proof hermetic seal between the adapter and the dry surface of the gel cassette, ensuring buffer integrity throughout the run.
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75 mL Upper Buffer Chamber: The geometry of the adapter is engineered to hold the exact volume of running buffer required for a Bio-Rad Criterion Cell, maintaining the consistent ionic environment necessary for sharp band resolution.
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Dual Alignment Tabs: Specifically designed to fit the mechanical slots of the Novex Midi Cassette, ensuring perfectly centered placement and a reliable fit every time.
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Disposable Styrene Construction: Manufactured from a rigid styrene copolymer that maintains its shape under the heat of electrophoresis while remaining cost-effective for single-use applications to prevent cross-contamination.
Product Specifications
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Catalog Number: WA0999
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Quantity: Pack of 10 Adapters
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Compatibility: Novex Midi Cassettes (NuPAGE or Tris-Glycine)
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Hardware Requirement: Bio-Rad Criterion Cell Electrophoresis Tank
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Dimensions: 13.7 cm (W) x 3.3 cm (H) x 2.8 cm (D)
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Buffer Capacity: ~75 mL
Key Benefits & Applications
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Cross-Platform Flexibility: Enables laboratories to switch to NuPAGE Midi Gels for superior protein stability and shelf life without the capital expense of purchasing a new Invitrogen gel tank.
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Leak Prevention: Replaces the need for makeshift gaskets or clamps; the self-adhesive design is the only manufacturer-validated method for running Invitrogen gels in Bio-Rad tanks.
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High-Resolution Protein Separation: By maintaining a stable upper buffer chamber, these adapters ensure uniform current flow across all lanes of the midi gel, which is critical for accurate Western blotting and mass spec preparation.
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Workflow Efficiency: The “peel-and-stick” application takes only seconds, allowing for a rapid transition from sample preparation to the start of the electrophoresis run.
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