Trypsin (0.25%) – EDTA (0.02%) w/ Phenol Red

Description

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Applications: Trypsin, 0.05% – EDTA, 0.1% assists in the dissociation of cell monolayers and tissue; most useful when dissociating fibroblasts and keratinocytes. While, Trypsin, 0.25% – EDTA, 0.02%, assists in detaching normal and transformed cells from vessel surfaces.