Description
Description
2-Mercaptoethanol For Molecular Biology For Electrophoresis 99% 500mL
Synonym:β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
- CAS Number 60-24-2
- Linear Formula HSCH2CH2OH
- Molecular Weight 78.13
- Beilstein/REAXYS Number 773648
- EC Number 200-464-6
- MDL number MFCD00004890
- PubChem Substance ID 24896800
- NACRES NA.31
| grade | for electrophoresis |
| for molecular biology | |
| vapor density | 2.69 (vs air) |
| vapor pressure | 1 mmHg ( 20 °C) |
| product line | BioReagent |
| assay | 99% (GC/titration) |
| expl. lim. | 18 % |
| concentration | 14.3 M (pure liquid) |
| application(s) | cell culture | mammalian: suitable |
| refractive index | n20/D 1.500 (lit.) |
| bp | 157 °C (lit.) |
| solubility | H2O: soluble 1 mL/mL |
| density | 1.114 g/mL at 25 °C (lit.) |
| foreign activity | DNase, RNase, protease, none detected |
| storage temp. | room temp |
| SMILES string | OCCS |
| InChI | 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2 |
| InChI key | DGVVWUTYPXICAM-UHFFFAOYSA-N |
Application
Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
GHS05, GHS06, GHS08, GHS09
Signal word Danger
Hazard statements H301 + H331-H310-H315-H317-H318-H373-H410
Precautionary statements P260-P262-P273-P280-P301 + P310 + P330-P302 + P352 + P310-P305 + P351 + P338 + P310-P391-P403 + P233
Personal Protective Equipment Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR UN 2966 6.1 / PGII
WGK Germany 3
RTECS KL5600000
Flash Point(F) 154.4 °F
Flash Point(C) 68 °C
Documents
Safety Data Sheet
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