Description
Description
Chaps Hydrate ≥98% HPLC 25 Gram
Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
- CAS Number 331717-45-4
- Empirical Formula (Hill Notation) C32H58N2O7S · xH2O
- Molecular Weight 614.88 (anhydrous basis)
- PubChem Substance ID 24892560
- NACRES NA.21
| Quality Level | 400 |
| description | zwitterionic |
| assay | ≥98% (HPLC) |
| mol wt | 614.88 g/mol (anhydrous basis) |
| application(s) | dialysis: suitable |
| electrophoresis: suitable | |
| CMC | 8 mM |
| mp | 157 °C (dec.) (lit.) |
| transition temp | cloud point >100 °C |
| SMILES string | O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C |
| InChI | 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1 |
| InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA-N |
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 – 10 mM) allowing it to be removed from samples by dialysis.
Application
0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.
Documents
Safety Data Sheet
Reviews
There are no reviews yet.