Description
Description
Chaps Hydrate Bioreagent ≥98% HPLC 5 Gram
Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
- CAS Number 331717-45-4
- Empirical Formula (Hill Notation) C32H58N2O7S · xH2O
- Molecular Weight 614.88 (anhydrous basis)
- PubChem Substance ID 24892997
- NACRES NB.22
| description | zwitterionic |
| product line | BioReagent |
| assay | ≥98% (HPLC) |
| mol wt | 614.88 g/mol (anhydrous basis) |
| application(s) | protein quantification: suitable |
| mp | 157 °C (dec.) (lit.) |
| suitability | suitable for electrophoresis |
| SMILES string | O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C |
| InChI | 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1 |
| InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA-N |
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 – 10 mM) allowing it to be removed from samples by dialysis.
Application
CHAPS has been used:
• For membrane permeabilization of cells
• As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
• As a component in denaturing buffer in enzyme characterization
• As a component of 2D- sample buffer in 2D electrophoresis
• For protein solubilization in isoelectric focusing
GHS08
Signal word Danger
Hazard statements H360
Precautionary statements P201-P280-P308 + P313
RIDADR NONH for all modes of transport
WGK Germany 1
Documents
Safety Data Sheet
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