Description
Description
Chloroform Biotech Grade ≥99.8% 0.5-1.0% Ethanol 2 Liter
Synonym: Methylidyne trichloride, Trichloromethane
- CAS Number 67-66-3
- Empirical Formula (Hill Notation) CHCl3
- Molecular Weight 119.38
- Beilstein/REAXYS Number 1731042
- MDL number MFCD00000826
- PubChem Substance ID 329757487
- NACRES NA.04
| grade | biotech. grade |
| vapor density | 4.1 (vs air) |
| vapor pressure | 160 mmHg ( 20 °C) |
| assay | ≥99.8% |
| contains | 0.5-1.0% ethanol as stabilizer |
| impurities | <0.01% water |
| evapn. residue | <0.0003% |
| color | APHA: ≤10 |
| refractive index | n20/D 1.445 (lit.) |
| pH | 5.2-6.4 |
| bp | 60.5-61.5 °C (lit.) |
| mp | −63 °C (lit.) |
| density | 1.48 g/mL at 25 °C |
| 1.492 g/mL at 25 °C (lit.) | |
| cation traces | Pb: ≤0.05 ppm |
| SMILES string | ClC(Cl)Cl |
| InChI | 1S/CHCl3/c2-1(3)4/h1H |
| InChI key | HEDRZPFGACZZDS-UHFFFAOYSA-N |
General description
Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Low water content, minimal residue and clean UV spectra are the special characteristics of this biotech grade solvent. It can be utilized for biotechnological processes like protein sequencing, peptide and oligonucleotide synthesis. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.
Application
Chloroform may be used in the following processes:
• Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).
• Preparing phospholipid solutions.
• As a solvent in the photolysis reaction of ruthenium complexes.
• Solvent for recrystallization.
• Reference compound to detect the presence of chloroform in Tobacco smokes.
• Extractant in solvent extraction process.
GHS06, GHS08
Signal word Danger
Hazard statements H302-H315-H319-H331-H336-H351-H361d-H372
Precautionary statements P201-P261-P304 + P340 + P312-P305 + P351 + P338-P308 + P313-P403 + P233
RIDADR UN 1888 6.1 / PGIII
WGK Germany 3
Documents
Safety Data Sheet





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