Description
Description
HEPES Bioperformance Certified ≥99.5% Titration 500 Gram
Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
- CAS Number 7365-45-9
- Empirical Formula (Hill Notation) C8H18N2O4S
- Molecular Weight 238.30
- Beilstein/REAXYS Number 883043
- EC Number 230-907-9
- MDL number MFCD00006158
- PubChem Substance ID 24895616
- NACRES NA.25
| Quality Level | 400 |
| grade | BioPerformance Certified |
| assay | ≥99.5% (titration) |
| application(s) | cell culture | mammalian: suitable |
| impurities | endotoxin and total aerobic microbial count, tested |
| useful pH range | 6.8 – 8.2 |
| pKa (25 °C) | 7.5 |
| cation traces | Fe: ≤5 ppm |
| absorption | ≤0.05 at 260 in H2O at 0.1 M |
| ≤0.05 at 280 in H2O at 0.1 M | |
| Featured Industry | Diagnostic Assay Manufacturing |
| foreign activity | DNase, RNase, NICKase, protease, none detected |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
General description
HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
• To supplement Dulbecco’s modified Eagle’s medium to culture and maintain cell lines
• As a component of platelet suspension buffer
• To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
• As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
• For the adjustment and maintenance of pH of biological solutions
• As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
• For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
• As a component of keratinocyte and fibroblast culture medium
Documents
Safety Data Sheet
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