Description
Description
Rpmi-1640 Mediumhepes Modification With 25 Mm Hepes Without L-Glutamine. Liquid Sterile-Filtered 500mL Case/6
- MDL number MFCD00217820
- NACRES NA.75
| Quality Level | 300 |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| pH range | >7.2 |
| components | L-glutamine: noNaHCO3: yessodium pyruvate: noHEPES: 25 mMphenol red: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
Application
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
Other Notes
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good’s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
Documents
Safety Data Sheet
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