Slide-A-Lyzer Dialysis Cassette G2 30 ml 10 kDa Lot of 3 Cassettes

Description

Thermo Scientific 87732 Slide-A-Lyzer G2 Dialysis Cassette (30 ml, 10K MWCO)

The Thermo Scientific 87732 is a high-performance, second-generation (G2) dialysis cassette designed for the rapid and secure salt removal, buffer exchange, and desalination of biological samples. Featuring a 30 ml capacity and a 10,000 MWCO regenerated cellulose membrane, this unit offers maximum surface-area-to-volume ratio for exceptionally fast dialysis kinetics. The G2 design is engineered for superior ease of use, utilizing a self-floating integrated air chamber and multiple access ports that allow for sample loading and retrieval using standard serological pipettes or tips—eliminating the risks associated with needles and syringes. This leak-proof, high-recovery vessel is the industry standard for processing sensitive proteins, nucleic acids, and complex carbohydrates.

Technical Performance and Design

• 10K MWCO Regenerated Cellulose: The high-purity membrane provides sharp molecular weight cut-offs and exceptionally low non-specific binding, ensuring >95% protein recovery even with dilute samples.

• Syringe-Free Loading: The G2 series features specialized ports that accommodate standard pipette tips. This allows for easy sample introduction and total withdrawal without the risk of membrane punctures or accidental needle sticks.

• Integrated Buoyancy: A built-in air chamber ensures the cassette floats vertically during the dialysis process. This orientation maximizes membrane exposure to the dialysis buffer and eliminates the need for external floats or clips.

• Fast Dialysis Kinetics: The dual-membrane design provides a massive surface area compared to traditional dialysis tubing, typically reducing a 24-hour dialysis process to just 4–8 hours for most applications.

Product Specifications

• Catalog Number: 87732

• Sample Volume Range: 10 ml to 30 ml

• MWCO: 10,000 Daltons (10K)

• Membrane Type: Regenerated Cellulose

• Cassette Format: G2 (Second Generation) with pipette-accessible ports

• Dimensions: Approximately 10 cm x 10 cm

• Packaging: Sold as individual cassettes or in packs of 8.

Key Benefits & Applications

• Buffer Exchange & Desalting: The most reliable method for removing salts, urea, or detergents from protein solutions prior to downstream analysis or storage.

• Protein Purification: Ideal for removing low-molecular-weight contaminants following chromatography or chemical modification (e.g., biotinylation or fluorescent labeling).

• Sample Concentration: Can be used in conjunction with a concentrating solution (like PEG) to reduce sample volume while maintaining the desired buffer environment.

• Structural Biology: Preferred for preparing high-purity protein samples for NMR or X-ray crystallography where buffer consistency is paramount.

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