Waters HPLC XSelect Column HSS T3 OBD 5 um 30 x 150 mm

Description

Waters XSelect HSS T3 Method Validation Kit / Prep Column – 30 mm ID x 150 mm Length, 5 µm Particle Size, OBD Preparative Technology, 100Å Pore (MPN: 186005790)

Used; excellent condition.

The Waters 186005790 is a high-performance preparative High-Performance Liquid Chromatography (Prep-HPLC) column engineered for the high-yield isolation, purification, and method validation of polar and non-polar organic compounds. Featuring substantial hardware dimensions of 30 mm Inner Diameter (ID) by 150 mm length packed with a 5-micrometer spherical High Strength Silica (HSS) particle matrix, this column utilizes Waters’ proprietary T3 bonding technology. This specialized low-density C18 chemistry delivers exceptional retention and selectivity for highly polar molecules under 100% aqueous mobile phase conditions, completely eliminating the risk of stationary phase collapse. Built with Optimum Bed Density (OBD) preparative technology, this system withstands extreme operational backpressures while maintaining excellent bed stability and preventing mechanical voids during continuous high-throughput separation passes.

Technical Performance and Design

• Advanced HSS T3 Polar Retention: Incorporates a unique, low-density C18 ligand bonding profile on an ultra-pure High Strength Silica substrate. This specialized surface chemistry allows polar analytes to interact effectively with the stationary phase, providing unmatched resolution and separation metrics without requiring ion-pairing additives.

• 100% Aqueous Phase Dewetting Defense: The T3 bonding architecture is explicitly designed to remain fully wetted and stable under 100 percent aqueous mobile phase environments. This feature eliminates the localized phase collapse (dewetting) common to traditional, densely packaged alkyl-bonded C18 columns.

• Optimum Bed Density (OBD) Packing: Packed using Waters’ proprietary OBD preparative technology. This process matches the mechanical density of the packed bed with the internal structural dynamics of the column hardware, ensuring excellent profile scalability from analytical scale up to preparative scale runs.

• High Strength Silica (HSS) Core: The ultra-pure, mechanically robust silica particle matrix is specifically engineered to handle the high linear velocities and intense backpressures of preparative fluid systems, extending the operational lifespan of the column packing bed.

Product Specifications

• Catalog Number / MPN: 186005790

• Manufacturer / Brand: Waters Corporation

• Column Chemistry / Phase: XSelect HSS T3 (High Strength Silica T3 C18)

• Internal Diameter (ID): 30 mm (Preparative Scale Dimensions)

• Column Length: 150 mm

• Particle Size Diameter: 5 µm (5 micrometers, spherical)

• Pore Size Metric: 100Å (100 Angstroms)

• Hardware Packing Technology: OBD (Optimum Bed Density) Preparative Design

• Carbon Load Matrix: Optimized ~11% carbon loading profile for balanced polar/non-polar selectivity

• pH Operational Envelope: 2.0 to 8.0 continuous working pH threshold

Key Benefits and Applications

• Preparative Sample Purification: The primary instrument tool utilized for scale-up isolation, fraction collection, and purification of active pharmaceutical ingredients (APIs), natural product extracts, and synthetic intermediates.

• Method Validation and Scale-Up: Designed to deliver flawless analytical-to-preparative scaling, allowing chemists to transfer analytical methods developed on smaller 5 µm particle profiles directly onto this 30 mm ID hardware with matching retention profiles.

• Water-Soluble Compound Analysis: Highly effective for separating challenging hydrophilic small molecules, including metabolites, amino acids, small peptides, and polar contaminants that elute too quickly on standard non-polar columns.

• Excellent Chemical Peak Symmetry: The advanced base-deactivation and endcapping processes minimize secondary silanol interactions, providing sharp peak shapes and low peak tailing for both acidic and basic target compounds.

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